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AlphaFold3 assessment

Interaction specificity · Confidence · Generalization

People

Océane Follonier, Yan Liu, Pablo Campomanes, Luc Lafrenaye, Julien Racle, Daniel Alvarez, Julian van Gerwen, Ricardo Heinzmann, Jürgen Jänes, Eric Kummelstedt, Janani Durairaj, David Gfeller, Stefano Vanni, Pedro Beltrao

Abstract

An assessment of AlphaFold3 across ubiquitinated proteins, TCR–epitope recognition, antibody–antigen complexes, protein–RNA and protein–lipid interactions. Performance varies by application; confidence does not consistently distinguish genuine interaction partners from decoys, and accuracy in several tasks depends on related complexes in the training set.